![]() ![]() These tools include gene expression analysis, primer design, molecular cloning, phylogenetic analyses, and more. For a comprehensive list of features and capabilities, visit the vendor website. Built upon the Genomics Workbench framework, this software has been optimized for use with samples from humans or a number of model organisms. To learn more, visit the vendor website.ĬLC Main Workbench contains a variety of toolkits to work with DNA, RNA, and protein analysis. ![]() Its main features include the ability to quickly analyze complex data, modify or personalize workflows, filter and visualize your data, and compare results with relevant databases. CLC Genomics Workbench supports key next-generation sequencing features within genomics, transcriptomics, and epigenomics, and includes all the tools of CLC Main Workbench. To learn more, visit the vendor website.ĬLC Biomedical Workbench offers flexible, ready-to-use analysis workflows. CLC Genomics Workbench incorporates cutting-edge technology and algorithms, while also supporting and integrating with the rest of a typical NGS workflow. Its user-friendly and intuitive interface essentially takes high-throughput analysis solely from bioinformatics programmers doing command-line scripts, and opens it up to scientists searching for biological results. CLC Genomics Workbench enables researchers to rapidly analyze and visualize large amounts of data generated by NGS machines. ![]() Citation: Juan Yang, Zhigang Han, Huifang Xu, Hui Xing, Peng Xu, Weibin Cheng. Given the mixing population, we suggest that a closed season be considered as an alternative strategy in managing the world s only freshwater sardine fishery.CLC Genomics Workbench is designed to solve the data analysis challenges of high-throughput sequencing with high-throughput sequencing machines. The sequences were edited with the software Sequencher (version 5.0. This suggests that all tawilis within Taal Lake are part of a single, panmictic stock. Rather, all specimens formed a single clade with no significant divergence between individuals from the eight fishing grounds for both genetic markers (Fig. ![]() Yet no fishing ground contained more than 45% of the total observed haplotype diversity and thus no ideal location for a new fish reserve could be identified. Results for the haplotype frequency analysis showed two dominant haplotypes to be common at most or all fishing locations, with northern basin locations of the lake supporting a higher mean and absolute number of haplotypes than locations in the southern basin. Sequences were cleaned using the genetics software Sequencher v4.8 (GeneCode Corp.), aligned and phylogenetic trees visualized in MEGA v5 (Tamura et al., 2007), and assessed for haplotype composition by location using DnaSP v5.1 (Librado and Rozas, 2009). Fish were indentified, the DNA extracted using a Chelex-resin method, amplified for the ribosomal 16S and mtDNA Cyt B gene regions, and sequenced. One hundred and forty-one specimens were collected from eight fishing locations around Taal Lake. This study applied molecular genetics tools to describe the phylogeographic pattern of tawilis across Taal Lake to determine whether a single or multiple stocks exist and which location supports the highest level of haplotype diversity to assist in the placement of the fish sanctuary. A proposed management strategy favors the demarcation of a new fish sanctuary however, it is unclear which area in the lake is appropriate for conservation. Locally referred to as tawilis, it is the most dominant and commercially important fishery of the lake, but in recent years severe depletion has been apparent in catch records. Sardinella tawilis (Clupeiformes: Clupeidae) is the world s only freshwater sardine and is endemic to Taal Lake, Batangas, Philippines. ![]()
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